Hotspot

rps16-trnQ-UUG

LSC · 6335-7224 · 890 bp

Back to Cercocarpus

Pi 0.00225

MarkerSeek score 62.40

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 3
pi: 0.00225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GTCGAATAGAAATGGGGCGT	50.0	50.0	58.0	58.1	1030-1031 bp	1.0	81.1
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	TCGAATAGAAATGGGGCGTG	50.0	50.0	58.0	58.1	1029-1030 bp	1.0	81.1
rps16-trnQ-UUG_p3	GTCTTTCAAGTCGCACGTTG	TGTCGAATAGAAATGGGGCG	50.0	50.0	58.0	58.1	1031-1032 bp	1.0	81.1
rps16-trnQ-UUG_p4	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	970-971 bp	1.0	81.0
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	GTCGAATAGAAATGGGGCGT	45.0	50.0	58.1	58.1	1023-1024 bp	1.0	80.0