Hotspot

rps3-rps19

LSC · 87358-87987 · 630 bp

Back to Cercis

Pi 0.0116
MarkerSeek score 66.20
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
630
variable_sites
11
pi
0.0116
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 GTTGCGCAAACCAAAGAGAA ACTCTAAACTTCCGGGGACA 45.0 50.0 57.8 58.0 757-847 bp 1.0 79.3
rps3-rps19_p2 TGCGCAAACCAAAGAGAATG ACTCTAAACTTCCGGGGACA 45.0 50.0 57.6 58.0 755-845 bp 1.0 77.6
rps3-rps19_p3 TACCAAGTCTGAAACCGAGC ACTCTAAACTTCCGGGGACA 50.0 50.0 57.5 58.0 721-811 bp 1.0 77.4
rps3-rps19_p4 GTCTGAAACCGAGCGGATTT ACTCTAAACTTCCGGGGACA 50.0 50.0 58.6 58.0 715-805 bp 1.0 76.5
rps3-rps19_p5 GCGCAAACCAAAGAGAATGA ACTCTAAACTTCCGGGGACA 45.0 50.0 57.3 58.0 754-844 bp 1.0 75.3