Hotspot

rps11-rpl36

LSC · 82979-83303 · 325 bp

Back to Cercis

Pi 0.0343
MarkerSeek score 63.90
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
325
variable_sites
7
pi
0.0343
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.42
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TACGCGCATTCTTACGTGAA AAAATAAGGGCCTCCGTTCG 45.0 50.0 58.0 58.3 315-506 bp 1.0 83.3
rps11-rpl36_p2 TTCTACGCGCATTCTTACGT AAAATAAGGGCCTCCGTTCG 45.0 50.0 57.7 58.3 318-509 bp 1.0 81.3
rps11-rpl36_p3 TACGCGCATTCTTACGTGAA AATAAGGGCCTCCGTTCGTA 45.0 50.0 58.0 58.5 313-504 bp 1.0 81.2
rps11-rpl36_p4 TACGCGCATTCTTACGTGAA ATAAGGGCCTCCGTTCGTAA 45.0 50.0 58.0 58.5 312-503 bp 1.0 81.2
rps11-rpl36_p5 TACGCGCATTCTTACGTGAA GGGCCTCCGTTCGTAAAATT 45.0 50.0 58.0 58.5 308-499 bp 1.0 81.0