Hotspot

accD-psaI

LSC · 61814-62710 · 897 bp

Back to Cercis

Pi 0.0169
MarkerSeek score 73.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
897
variable_sites
22
pi
0.0169
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GAAGGTTCACAAGCGTCTGA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.1 58.0 1065-1090 bp 1.0 83.0
accD-psaI_p2 GAAGGTTCACAAGCGTCTGA AAGGAAGCCATTGCAATTGC 50.0 45.0 58.1 57.9 1072-1097 bp 1.0 82.3
accD-psaI_p3 AAGGTTCACAAGCGTCTGAA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.7 58.0 1064-1089 bp 1.0 81.4
accD-psaI_p4 AGGGCGTTCTGAGTGAGTTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.4 58.0 992-1017 bp 1.0 81.4
accD-psaI_p5 AAGGTTCACAAGCGTCTGAA AAGGAAGCCATTGCAATTGC 45.0 45.0 57.7 57.9 1071-1096 bp 1.0 80.8