Hotspot

psbZ-trnG-GCC

LSC · 38150-38657 · 508 bp

Back to Cercis

Pi 0.00947
MarkerSeek score 64.90
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
508
variable_sites
7
pi
0.00947
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CGTTGTATTTGCTTCGCCAG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.1 57.8 672-697 bp 1.0 81.0
psbZ-trnG-GCC_p2 TATTTGCTTCGCCAGATGGT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.6 57.8 667-692 bp 1.0 78.6
psbZ-trnG-GCC_p3 ATTTGCTTCGCCAGATGGTT CGTCTTCTCCTTGGCAAAGA 45.0 50.0 58.5 57.8 666-691 bp 1.0 78.4
psbZ-trnG-GCC_p4 GTATTTGCTTCGCCAGATGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.2 57.8 668-693 bp 1.0 75.8
psbZ-trnG-GCC_p5 TTTGCTTCGCCAGATGGTT CGTCTTCTCCTTGGCAAAGA 47.4 50.0 58.0 57.8 665-690 bp 1.0 73.7