Hotspot

trnT-GGU-psbD

LSC · 33122-34856 · 1735 bp

Back to Cercis

Pi 0.0143
MarkerSeek score 70.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1735
variable_sites
36
pi
0.0143
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1899-1934 bp 1.0 80.8
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GACCGGACCAACCTACAAAA 50.0 50.0 57.9 57.7 1913-1948 bp 1.0 80.7
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA TAGACCGGACCAACCTACAA 50.0 50.0 57.9 57.7 1915-1950 bp 1.0 80.3
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GAGCAATAGACCGGACCAAC 50.0 55.0 57.9 58.3 1921-1956 bp 1.0 80.0
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA CAAAAACGAAACGGTCCCTC 50.0 50.0 57.9 57.6 1898-1933 bp 1.0 79.6