Hotspot

petN-psbM

LSC · 29480-30335 · 856 bp

Back to Cercis

Pi 0.00806
MarkerSeek score 66.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
856
variable_sites
10
pi
0.00806
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT AGTTCCTACTGCCTTTTTGCT 50.0 42.9 58.2 57.8 968-997 bp 1.0 72.1
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTCT 50.0 42.9 58.2 57.6 989-1018 bp 1.0 70.7
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT AGTTCCTACTGCCTTTTTGC 50.0 45.0 58.2 56.2 968-997 bp 1.0 67.9
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT GTTCCTACTGCCTTTTTGCT 50.0 45.0 58.2 56.2 967-996 bp 1.0 67.9
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTC 50.0 45.0 58.2 56.1 989-1018 bp 1.0 66.5