Hotspot

rpoB-trnC-GCA

LSC · 27383-28561 · 1179 bp

Back to Cercis

Pi 0.00966
MarkerSeek score 52.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1179
variable_sites
14
pi
0.00966
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCGCCTACACAAGCAAATTG AGACCCAGTCAGTAAACGGA 50.0 50.0 58.0 58.0 2209-2227 bp 1.0 81.1
rpoB-trnC-GCA_p2 CGAGCCCATATCCTTGCTTT AGACCCAGTCAGTAAACGGA 50.0 50.0 58.0 58.0 2017-2035 bp 1.0 81.1
rpoB-trnC-GCA_p3 CCGCCTACACAAGCAAATTG CCGACCTATCCCTACCCAAT 50.0 55.0 58.0 58.0 2237-2255 bp 1.0 80.9
rpoB-trnC-GCA_p4 CGAGCCCATATCCTTGCTTT CCGACCTATCCCTACCCAAT 50.0 55.0 58.0 58.0 2045-2063 bp 1.0 80.9
rpoB-trnC-GCA_p5 AGACATTCCCTCATTTCCGC AGACCCAGTCAGTAAACGGA 50.0 50.0 58.0 58.0 1535-1553 bp 0.666667 67.6