Hotspot

atpH-atpI

LSC · 14139-15126 · 988 bp

Back to Cercis

Pi 0.00207
MarkerSeek score 62.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
988
variable_sites
3
pi
0.00207
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 GCCAATCCAGCAGCAATAAC TTTGCAACTTTAGCTGCAGC 50.0 45.0 57.8 57.9 1067-1089 bp 1.0 79.2
atpH-atpI_p2 GCCAATCCAGCAGCAATAAC CGAATCCATGGAGGGTCATC 50.0 55.0 57.8 57.8 1038-1060 bp 1.0 78.9
atpH-atpI_p3 TCCAGCAGCAATAACGGAAG TTTGCAACTTTAGCTGCAGC 50.0 45.0 58.3 57.9 1062-1084 bp 1.0 78.8
atpH-atpI_p4 CCTTCTACAGCTTGACCTGC TTTGCAACTTTAGCTGCAGC 55.0 45.0 58.3 57.9 1133-1155 bp 1.0 78.6
atpH-atpI_p5 TCCAGCAGCAATAACGGAAG CGAATCCATGGAGGGTCATC 50.0 55.0 58.3 57.8 1033-1055 bp 1.0 78.6