Hotspot

atpF-atpH

LSC · 13241-13892 · 652 bp

Back to Cercis

Pi 0.019
MarkerSeek score 73.90
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
652
variable_sites
15
pi
0.019
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CGGATGTCCAGTAACCCAAG AGGCAGAGGGAAAAATACGG 55.0 50.0 58.0 57.6 778-817 bp 1.0 82.0
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG AGGCAGAGGGAAAAATACGG 50.0 50.0 57.9 57.6 798-837 bp 1.0 81.2
atpF-atpH_p3 CGGATGTCCAGTAACCCAAG GCAGAGGGAAAAATACGGGG 55.0 55.0 58.0 58.6 776-815 bp 1.0 80.4
atpF-atpH_p4 CGGATGTCCAGTAACCCAAG GGCAGAGGGAAAAATACGGG 55.0 55.0 58.0 58.6 777-816 bp 1.0 80.4
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG GCAGAGGGAAAAATACGGGG 50.0 55.0 57.9 58.6 796-835 bp 1.0 79.6