Hotspot

trnR-UCU-atpA

LSC · 9995-10357 · 363 bp

Back to Cercis

Pi 0.00682
MarkerSeek score 61.70
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
363
variable_sites
3
pi
0.00682
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGAGAAAAGCGTCCATTGTCT TGAAGCAGAAGCCCTTTTGA 42.9 45.0 57.6 57.6 509-525 bp 1.0 70.3
trnR-UCU-atpA_p2 AGAGAAAAGCGTCCATTGTCT GTGAAGCAGAAGCCCTTTTG 42.9 50.0 57.6 57.6 510-526 bp 1.0 69.8
trnR-UCU-atpA_p3 AGAGAAAAGCGTCCATTGTCT TACCAAGACATTCACGGGTG 42.9 50.0 57.6 57.5 527-543 bp 1.0 69.5
trnR-UCU-atpA_p4 GGTTCAAATCCTATTGGACGC TGAAGCAGAAGCCCTTTTGA 47.6 45.0 57.3 57.6 451-467 bp 1.0 68.1
trnR-UCU-atpA_p5 AGAGAAAAGCGTCCATTGTCT GGTGAAGCAGAAGCCCTTTT 42.9 50.0 57.6 58.7 511-527 bp 1.0 67.8