primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCACTTAAAAGCCGAG	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.6	58.0	6.4	7.6	0.594878	4298	5053	899-914	1.000000	76.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GAGTCGCACTTAAAAGCCGA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.6	58.0	3.0	7.6	0.598928	4298	5053	900-915	1.000000	76.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.3	58.0	3.0	7.6	0.753030	4298	5053	897-912	1.000000	75.1
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCACTTAAAAGCCGAG	CCTTGAAAAAGGCGCTCAAC	20	20	50.0	50.0	58.6	58.2	6.4	7.6	0.799581	4298	5053	904-919	1.000000	74.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.6	58.2	6.4	7.6	0.799581	4298	5053	903-918	1.000000	74.7
trnS-GCU-trnR-UCU_p1	trnS-GCU-trnR-UCU	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	8025	9922	1937-1969	1.000000	67.9
trnS-GCU-trnR-UCU_p2	trnS-GCU-trnR-UCU	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	8025	9922	1936-1968	1.000000	67.9
trnS-GCU-trnR-UCU_p3	trnS-GCU-trnR-UCU	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	8025	9922	1943-1975	1.000000	67.2
trnS-GCU-trnR-UCU_p4	trnS-GCU-trnR-UCU	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	8025	9922	1942-1974	1.000000	67.2
trnS-GCU-trnR-UCU_p5	trnS-GCU-trnR-UCU	5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.2	56.9	0.0	0.0	2.202953	8025	9922	1991-2023	1.000000	66.6
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	AGAGAAAAGCGTCCATTGTCT	TGAAGCAGAAGCCCTTTTGA	21	20	42.9	45.0	57.6	57.6	0.0	3.1	1.772333	9995	10357	509-525	1.000000	70.3
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	AGAGAAAAGCGTCCATTGTCT	GTGAAGCAGAAGCCCTTTTG	21	20	42.9	50.0	57.6	57.6	0.0	0.0	1.837914	9995	10357	510-526	1.000000	69.8
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	AGAGAAAAGCGTCCATTGTCT	TACCAAGACATTCACGGGTG	21	20	42.9	50.0	57.6	57.5	0.0	0.0	1.874887	9995	10357	527-543	1.000000	69.5
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	TGAAGCAGAAGCCCTTTTGA	21	20	47.6	45.0	57.3	57.6	0.0	3.1	2.056097	9995	10357	451-467	1.000000	68.1
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	AGAGAAAAGCGTCCATTGTCT	GGTGAAGCAGAAGCCCTTTT	21	20	42.9	50.0	57.6	58.7	0.0	0.0	2.076832	9995	10357	511-527	1.000000	67.8
atpF-atpH_p1	atpF-atpH	1	CGGATGTCCAGTAACCCAAG	AGGCAGAGGGAAAAATACGG	20	20	55.0	50.0	58.0	57.6	0.0	0.0	0.444234	13241	13892	778-817	1.000000	82.0
atpF-atpH_p2	atpF-atpH	2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.538372	13241	13892	798-837	1.000000	81.2
atpF-atpH_p3	atpF-atpH	3	CGGATGTCCAGTAACCCAAG	GCAGAGGGAAAAATACGGGG	20	20	55.0	55.0	58.0	58.6	0.0	0.0	0.634620	13241	13892	776-815	1.000000	80.4
atpF-atpH_p4	atpF-atpH	4	CGGATGTCCAGTAACCCAAG	GGCAGAGGGAAAAATACGGG	20	20	55.0	55.0	58.0	58.6	0.0	0.0	0.634620	13241	13892	777-816	1.000000	80.4
atpF-atpH_p5	atpF-atpH	5	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	20	20	50.0	55.0	57.9	58.6	0.0	0.0	0.728758	13241	13892	796-835	1.000000	79.6
atpH-atpI_p1	atpH-atpI	1	GCCAATCCAGCAGCAATAAC	TTTGCAACTTTAGCTGCAGC	20	20	50.0	45.0	57.8	57.9	0.0	31.5	0.352983	14139	15126	1067-1089	1.000000	79.2
atpH-atpI_p2	atpH-atpI	2	GCCAATCCAGCAGCAATAAC	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.390265	14139	15126	1038-1060	1.000000	78.9
atpH-atpI_p3	atpH-atpI	3	TCCAGCAGCAATAACGGAAG	TTTGCAACTTTAGCTGCAGC	20	20	50.0	45.0	58.3	57.9	0.0	31.5	0.400758	14139	15126	1062-1084	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	CCTTCTACAGCTTGACCTGC	TTTGCAACTTTAGCTGCAGC	20	20	55.0	45.0	58.3	57.9	0.0	31.5	0.406876	14139	15126	1133-1155	1.000000	78.6
atpH-atpI_p5	atpH-atpI	5	TCCAGCAGCAATAACGGAAG	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	58.3	57.8	0.0	21.3	0.438039	14139	15126	1033-1055	1.000000	78.6
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCGCCTACACAAGCAAATTG	AGACCCAGTCAGTAAACGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.024117	27383	28561	2209-2227	1.000000	81.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	AGACCCAGTCAGTAAACGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032498	27383	28561	2017-2035	1.000000	81.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCGCCTACACAAGCAAATTG	CCGACCTATCCCTACCCAAT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.044382	27383	28561	2237-2255	1.000000	80.9
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CGAGCCCATATCCTTGCTTT	CCGACCTATCCCTACCCAAT	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.052764	27383	28561	2045-2063	1.000000	80.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	AGACATTCCCTCATTTCCGC	AGACCCAGTCAGTAAACGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.049493	27383	28561	1535-1553	0.666667	67.6
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCCTTTTTGCT	20	21	50.0	42.9	58.2	57.8	0.0	0.0	1.381045	29480	30335	968-997	1.000000	72.1
petN-psbM_p2	petN-psbM	2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.2	57.6	0.0	0.0	1.558772	29480	30335	989-1018	1.000000	70.7
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	AGTTCCTACTGCCTTTTTGC	20	20	50.0	45.0	58.2	56.2	0.0	0.0	1.916619	29480	30335	968-997	1.000000	67.9
petN-psbM_p4	petN-psbM	4	TAGTAAGTCTCGCTTGGGCT	GTTCCTACTGCCTTTTTGCT	20	20	50.0	45.0	58.2	56.2	0.0	0.0	1.916619	29480	30335	967-996	1.000000	67.9
petN-psbM_p5	petN-psbM	5	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.2	56.1	0.0	0.0	2.081320	29480	30335	989-1018	1.000000	66.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.349607	33122	34856	1899-1934	1.000000	80.8
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GCCCTTTTAACTCAGCGGTA	GACCGGACCAACCTACAAAA	20	20	50.0	50.0	57.9	57.7	0.0	9.5	0.362803	33122	34856	1913-1948	1.000000	80.7
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GCCCTTTTAACTCAGCGGTA	TAGACCGGACCAACCTACAA	20	20	50.0	50.0	57.9	57.7	0.0	9.5	0.411033	33122	34856	1915-1950	1.000000	80.3
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	GAGCAATAGACCGGACCAAC	20	20	50.0	55.0	57.9	58.3	0.0	9.5	0.450386	33122	34856	1921-1956	1.000000	80.0
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GCCCTTTTAACTCAGCGGTA	CAAAAACGAAACGGTCCCTC	20	20	50.0	50.0	57.9	57.6	0.0	0.0	0.498082	33122	34856	1898-1933	1.000000	79.6
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CGTTGTATTTGCTTCGCCAG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	1.1	0.287634	38150	38657	672-697	1.000000	81.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TATTTGCTTCGCCAGATGGT	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	57.6	57.8	0.0	1.1	0.588200	38150	38657	667-692	1.000000	78.6
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	ATTTGCTTCGCCAGATGGTT	CGTCTTCTCCTTGGCAAAGA	20	20	45.0	50.0	58.5	57.8	0.0	1.1	0.620629	38150	38657	666-691	1.000000	78.4
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	GTATTTGCTTCGCCAGATGG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	57.2	57.8	0.0	1.1	0.933081	38150	38657	668-693	1.000000	75.8
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTTGCTTCGCCAGATGGTT	CGTCTTCTCCTTGGCAAAGA	19	20	47.4	50.0	58.0	57.8	0.0	1.1	1.204757	38150	38657	665-690	1.000000	73.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48592	50056	1515-1549	1.000000	83.4
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48592	50056	1523-1557	1.000000	82.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48592	50056	1547-1581	1.000000	82.6
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	48592	50056	1555-1589	1.000000	82.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	48592	50056	1529-1563	1.000000	81.5
accD-psaI_p1	accD-psaI	1	GAAGGTTCACAAGCGTCTGA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.161138	61814	62710	1065-1090	1.000000	83.0
accD-psaI_p2	accD-psaI	2	GAAGGTTCACAAGCGTCTGA	AAGGAAGCCATTGCAATTGC	20	20	50.0	45.0	58.1	57.9	0.0	24.1	0.237672	61814	62710	1072-1097	1.000000	82.3
accD-psaI_p3	accD-psaI	3	AAGGTTCACAAGCGTCTGAA	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.352586	61814	62710	1064-1089	1.000000	81.4
accD-psaI_p4	accD-psaI	4	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.4	58.0	0.0	26.9	0.397961	61814	62710	992-1017	1.000000	81.4
accD-psaI_p5	accD-psaI	5	AAGGTTCACAAGCGTCTGAA	AAGGAAGCCATTGCAATTGC	20	20	45.0	45.0	57.7	57.9	0.0	24.1	0.429120	61814	62710	1071-1096	1.000000	80.8
rps11-rpl36_p1	rps11-rpl36	1	TACGCGCATTCTTACGTGAA	AAAATAAGGGCCTCCGTTCG	20	20	45.0	50.0	58.0	58.3	5.7	6.5	0.273633	82979	83303	315-506	1.000000	83.3
rps11-rpl36_p2	rps11-rpl36	2	TTCTACGCGCATTCTTACGT	AAAATAAGGGCCTCCGTTCG	20	20	45.0	50.0	57.7	58.3	5.7	6.5	0.520718	82979	83303	318-509	1.000000	81.3
rps11-rpl36_p3	rps11-rpl36	3	TACGCGCATTCTTACGTGAA	AATAAGGGCCTCCGTTCGTA	20	20	45.0	50.0	58.0	58.5	5.7	6.5	0.531713	82979	83303	313-504	1.000000	81.2
rps11-rpl36_p4	rps11-rpl36	4	TACGCGCATTCTTACGTGAA	ATAAGGGCCTCCGTTCGTAA	20	20	45.0	50.0	58.0	58.5	5.7	6.5	0.531713	82979	83303	312-503	1.000000	81.2
rps11-rpl36_p5	rps11-rpl36	5	TACGCGCATTCTTACGTGAA	GGGCCTCCGTTCGTAAAATT	20	20	45.0	50.0	58.0	58.5	5.7	0.8	0.563807	82979	83303	308-499	1.000000	81.0
rps3-rps19_p1	rps3-rps19	1	GTTGCGCAAACCAAAGAGAA	ACTCTAAACTTCCGGGGACA	20	20	45.0	50.0	57.8	58.0	26.9	0.0	0.222103	87358	87987	757-847	1.000000	79.3
rps3-rps19_p2	rps3-rps19	2	TGCGCAAACCAAAGAGAATG	ACTCTAAACTTCCGGGGACA	20	20	45.0	50.0	57.6	58.0	8.2	0.0	0.440436	87358	87987	755-845	1.000000	77.6
rps3-rps19_p3	rps3-rps19	3	TACCAAGTCTGAAACCGAGC	ACTCTAAACTTCCGGGGACA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.470417	87358	87987	721-811	1.000000	77.4
rps3-rps19_p4	rps3-rps19	4	GTCTGAAACCGAGCGGATTT	ACTCTAAACTTCCGGGGACA	20	20	50.0	50.0	58.6	58.0	16.8	0.0	0.580129	87358	87987	715-805	1.000000	76.5
rps3-rps19_p5	rps3-rps19	5	GCGCAAACCAAAGAGAATGA	ACTCTAAACTTCCGGGGACA	20	20	45.0	50.0	57.3	58.0	0.3	0.0	0.724533	87358	87987	754-844	1.000000	75.3
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	CGAACCCTTCCATTCCGAAT	CAAATATGTAGGGGGTGGCC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.043011	113000	114295	1324-1518	1.000000	87.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	ACGACCAGTCAGTTAACAGC	CAAATATGTAGGGGGTGGCC	20	20	50.0	55.0	57.8	58.0	8.6	0.0	0.155021	113000	114295	1257-1451	1.000000	86.3
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	AGGATTCGAACCTACGACCA	CAAATATGTAGGGGGTGGCC	20	20	50.0	55.0	58.2	58.0	15.0	0.0	0.161255	113000	114295	1270-1464	1.000000	86.2
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GGATTCGAACCTACGACCAG	CAAATATGTAGGGGGTGGCC	20	20	55.0	55.0	57.8	58.0	0.0	0.0	0.209930	113000	114295	1269-1463	1.000000	85.8
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	CCCCAAGTAGGATTCGAACC	CAAATATGTAGGGGGTGGCC	20	20	55.0	55.0	57.7	58.0	0.0	0.0	0.331824	113000	114295	1278-1472	1.000000	84.8
ndhF-rpl32_p1	ndhF-rpl32	1	TGTTGCCGTCGGAAAAAGTA	AAAGCTTTCAATGCTGTCCA	20	20	45.0	40.0	58.0	56.1	17.5	5.7	1.916660	116552	117583	1226-1256	1.000000	66.7
ndhF-rpl32_p2	ndhF-rpl32	2	TGTTGCCGTCGGAAAAAGTA	AAGCTTTCAATGCTGTCCAA	20	20	45.0	40.0	58.0	56.1	17.5	0.0	1.916660	116552	117583	1225-1255	1.000000	66.7
ndhF-rpl32_p3	ndhF-rpl32	3	TTTGTTGCCGTCGGAAAAAG	AAAGCTTTCAATGCTGTCCA	20	20	45.0	40.0	57.8	56.1	17.5	5.7	2.063783	116552	117583	1228-1258	1.000000	65.5
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCCGTCGGAAAAAG	AAGCTTTCAATGCTGTCCAA	20	20	45.0	40.0	57.8	56.1	17.5	0.0	2.063783	116552	117583	1227-1257	1.000000	65.5
ndhF-rpl32_p5	ndhF-rpl32	5	TGTTGCCGTCGGAAAAAGTA	ACGAAAAAGCTTTCAATGCTGT	20	22	45.0	36.4	58.0	58.0	17.5	13.1	2.094808	116552	117583	1231-1261	1.000000	65.2
ndhD-psaC_p1	ndhD-psaC	1	TGGATCGTCCAATTGGAAATGA	AGAGGACTGTGTCGGTTGTA	22	20	40.9	50.0	58.0	58.0	28.6	0.0	2.047030	121089	121340	421-422	1.000000	65.5
ndhD-psaC_p2	ndhD-psaC	2	TGGATCGTCCAATTGGAAATGA	CCGACGGATTTCTTGAGTGT	22	20	40.9	50.0	58.0	57.9	28.6	8.6	2.118437	121089	121340	381-382	1.000000	65.2
ndhD-psaC_p3	ndhD-psaC	3	TCCGTCAGTTGGATTAATGGA	AGAGGACTGTGTCGGTTGTA	21	20	42.9	50.0	56.7	58.0	0.0	0.0	2.281173	121089	121340	438-439	1.000000	63.6
ndhD-psaC_p4	ndhD-psaC	4	TCCGTCAGTTGGATTAATGGA	CCGACGGATTTCTTGAGTGT	21	20	42.9	50.0	56.7	57.9	0.0	8.6	2.352580	121089	121340	398-399	1.000000	63.2
ndhD-psaC_p5	ndhD-psaC	5	TGGATCGTCCAATTGGAAATGA	AAGAGATGTGAATCCGCCTG	22	20	40.9	50.0	58.0	57.7	28.6	0.0	2.360506	121089	121340	402-403	1.000000	63.1
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.145058	122934	123408	619-686	1.000000	81.9
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	57.9	57.9	0.0	0.0	0.157730	122934	123408	682-749	1.000000	81.5
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCGATCCCAGAAAGA	TTTGGGCCGTTTACCAATGT	20	20	50.0	45.0	58.0	58.3	0.0	0.0	0.341506	122934	123408	608-675	1.000000	80.4
ndhG-ndhI_p4	ndhG-ndhI	4	TCCCAACGAAAAGGCAGAAA	TTTGGGCCGTTTACCAATGT	20	20	45.0	45.0	57.9	58.3	0.0	0.0	0.354177	122934	123408	671-738	1.000000	80.0
ndhG-ndhI_p5	ndhG-ndhI	5	AAGACCCGATCCCAGAAAGA	CTTTGGGCCGTTTACCAATG	20	20	50.0	50.0	58.0	57.6	0.0	0.0	0.409548	122934	123408	609-676	1.000000	79.8