Hotspot

ycf1

SSC · 149512-155148 · 5637 bp

Back to Cercidiphyllum

Pi 0.00177

MarkerSeek score 51.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5637
variable_sites: 10
pi: 0.00177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGTATCCCAGGCTTCTGCTA	CGCGCTCAAAGACGTAAAAC	55.0	50.0	58.6	58.4	282 bp	1.0	72.9
ycf1_p2	TCCCAGGCTTCTGCTATTTC	CGCGCTCAAAGACGTAAAAC	50.0	50.0	57.3	58.4	278 bp	1.0	71.7
ycf1_p3	CCCAGGCTTCTGCTATTTCT	CGCGCTCAAAGACGTAAAAC	50.0	50.0	57.3	58.4	277 bp	1.0	71.7
ycf1_p4	ACAATGGTATCCCAGGCTTC	CGCGCTCAAAGACGTAAAAC	50.0	50.0	57.3	58.4	287 bp	1.0	71.6
ycf1_p5	GGTATCCCAGGCTTCTGCTA	GCGCTCAAAGACGTAAAACA	55.0	45.0	58.6	57.3	281 bp	1.0	71.0