Hotspot

rbcL

LSC · 79095-80522 · 1428 bp

Back to Cercidiphyllum

Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 2
pi: 0.0014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1500 bp	1.0	71.4
rbcL_p2	TGAGTTGTAGGGAGGGACTT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1501 bp	1.0	71.4
rbcL_p3	TCATGAGTTGTAGGGAGGGA	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.1	58.4	1503 bp	1.0	69.6
rbcL_p4	TCATGAGTTGTAGGGAGGGA	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.1	58.4	1504 bp	1.0	69.6
rbcL_p5	ACGCCTAATTCATGTCGAGT	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.0	58.4	1551 bp	1.0	69.4