Hotspot

accD-psaI

LSC · 82681-83429 · 749 bp

Back to Cercidiphyllum

Pi 0.00267

MarkerSeek score 60.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 2
pi: 0.00267
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAGGCGTTCTGAGTGAGT	GGCACCAAAACAGAGGGTAA	45.0	50.0	57.4	58.0	854-870 bp	1.0	75.1
accD-psaI_p2	AAAGGCGTTCTGAGTGAGTT	GGCACCAAAACAGAGGGTAA	45.0	50.0	57.4	58.0	853-869 bp	1.0	75.1
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	GGCACCAAAACAGAGGGTAA	50.0	50.0	58.8	58.0	827-843 bp	1.0	74.0
accD-psaI_p4	AAAGGCGTTCTGAGTGAGTT	AAACCATTGCTATTGCCGGA	45.0	45.0	57.4	58.2	891-907 bp	1.0	74.0
accD-psaI_p5	AAAGGCGTTCTGAGTGAGTT	AACCATTGCTATTGCCGGAA	45.0	45.0	57.4	58.2	890-906 bp	1.0	74.0