Hotspot

atpB-rbcL

LSC · 78338-79094 · 757 bp

Back to Cercidiphyllum

Pi 0.00133
MarkerSeek score 60.20
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
757
variable_sites
1
pi
0.00133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 CATCCAGTACCGGACCAATG CACCAGCTTTGAATCCAACAC 55.0 47.6 58.0 58.0 898-903 bp 1.0 72.0
atpB-rbcL_p2 GGGGGAAAAGCTACATCCAG CACCAGCTTTGAATCCAACAC 55.0 47.6 57.9 58.0 911-916 bp 1.0 71.9
atpB-rbcL_p3 CATCCAGTACCGGACCAATG ACCAGCTTTGAATCCAACACT 55.0 42.9 58.0 58.1 897-902 bp 1.0 71.8
atpB-rbcL_p4 GGGGGAAAAGCTACATCCAG ACCAGCTTTGAATCCAACACT 55.0 42.9 57.9 58.1 910-915 bp 1.0 71.7
atpB-rbcL_p5 TCCAGTACCGGACCAATGAT CACCAGCTTTGAATCCAACAC 50.0 47.6 58.1 58.0 896-901 bp 1.0 71.4