Hotspot

rps4-trnT-UGU

LSC · 70132-70476 · 345 bp

Back to Cercidiphyllum

Pi 0.0058

MarkerSeek score 56.80

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 2
pi: 0.0058
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	457 bp	1.0	77.2
rps4-trnT-UGU_p2	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	503 bp	1.0	76.8
rps4-trnT-UGU_p3	GTTAGTCCCGGTAAAGCTCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.7	57.9	473 bp	1.0	76.8
rps4-trnT-UGU_p4	CTCCCAGACGGCGTATTTTT	GGTTCGATTCCGATAGCTGG	50.0	55.0	58.3	58.2	416 bp	1.0	76.5
rps4-trnT-UGU_p5	CTCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	425 bp	1.0	76.3