Hotspot

psaA-ycf3

LSC · 65713-66394 · 682 bp

Back to Cercidiphyllum

Pi 0

MarkerSeek score 60.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 682
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTTCTTGAGAAATGGCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	871-885 bp	1.0	77.2
psaA-ycf3_p2	TTTTACTTCTGGTTCCGGCG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.5	58.0	799-813 bp	1.0	77.2
psaA-ycf3_p3	TGTTCTTGAGAAATGGCCGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.5	58.1	873-887 bp	1.0	76.4
psaA-ycf3_p4	TTTTACTTCTGGTTCCGGCG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.5	58.1	801-815 bp	1.0	76.4
psaA-ycf3_p5	TGTTCTTGAGAAATGGCCGG	AGATCACGAGGCGTTTTGAA	50.0	45.0	58.5	57.8	796-810 bp	1.0	76.4