Hotspot

psbZ-trnG-UCC

LSC · 59829-60313 · 485 bp

Back to Cercidiphyllum

Pi 0.0104

MarkerSeek score 62.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 485
variable_sites: 5
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	642-700 bp	1.0	78.0
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.3	57.8	640-698 bp	1.0	77.5
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	CATTACTTTACCCTGGGCGG	50.0	55.0	57.8	58.3	681-739 bp	1.0	76.7
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTC	CATTACTTTACCCTGGGCGG	55.0	55.0	58.3	58.3	679-737 bp	1.0	76.3
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	TTACTTTACCCTGGGCGGAT	50.0	50.0	57.8	58.4	679-737 bp	1.0	76.0