Hotspot

petN-trnD-GUC

LSC · 51061-53512 · 2452 bp

Back to Cercidiphyllum

Pi 0.00122

MarkerSeek score 57.50

Primer available yes

Label petN-trnD-GUC

Feature Metadata

label_name: petN-trnD-GUC
feature_type: igs
region: LSC
length_bp: 2452
variable_sites: 3
pi: 0.00122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnD-GUC_p1	TAGTAAGTCTCGCTTGGGCT	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.2	58.5	2599-2600 bp	1.0	75.1
petN-trnD-GUC_p2	ATGGGGAAGAAGTGGACTCT	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.6	58.5	2538-2539 bp	1.0	73.5
petN-trnD-GUC_p3	TAGTAAGTCTCGCTTGGGCT	GTTCAATTGGTCAGAGCACC	50.0	50.0	58.2	57.0	2600-2601 bp	1.0	70.9
petN-trnD-GUC_p4	ATGGGGAAGAAGTGGACTCT	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.6	57.0	2539-2540 bp	1.0	69.3
petN-trnD-GUC_p5	ATAGTAAGTCTCGCTTGGGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.1	58.5	2600-2601 bp	1.0	69.1