Hotspot

ycf3

LSC · 57306-59708 · 2403 bp

Back to Ceratostigma

Pi 0.0169

MarkerSeek score 61.80

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2403
variable_sites: 57
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TCCCACGAGAGGTAATGAGA	TGATTGATTGGCTCTTCCCG	50.0	50.0	57.2	58.0	2458-2510 bp	1.0	78.9
ycf3_p2	TCCCACGAGAGGTAATGAGA	GATTGGCTCTTCCCGGAATT	50.0	50.0	57.2	57.9	2453-2505 bp	1.0	78.8
ycf3_p3	TCCCACGAGAGGTAATGAGA	TTGGCTCTTCCCGGAATTTT	50.0	45.0	57.2	57.7	2451-2503 bp	1.0	76.9
ycf3_p4	TCCCACGAGAGGTAATGAGAA	TGATTGATTGGCTCTTCCCG	47.6	50.0	57.9	58.0	2458-2510 bp	1.0	76.7
ycf3_p5	TTCCCACGAGAGGTAATGAGA	TGATTGATTGGCTCTTCCCG	47.6	50.0	57.9	58.0	2459-2511 bp	1.0	76.7