Hotspot

ycf1

IRa · 134065-141612 · 7548 bp

Back to Ceratostigma

Pi 0.00716

MarkerSeek score 45.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRa
length_bp: 7548
variable_sites: 77
pi: 0.00716
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGCTCCAATTCTTCCTTCT	TCAAAAGCAAGAAAAGCAAGA	50.0	33.3	57.6	54.8	289-328 bp	1.0	51.2
ycf1_p2	GCAAAGCGTATAGTCTCCCA	TCAAAAGCAAGAAAAGCAAGA	50.0	33.3	57.4	54.8	257-296 bp	1.0	50.2
ycf1_p3	CGGCTCCAATTCTTCCTTCT	TCAAAAGCAAGAAAAGCAAGAA	50.0	31.8	57.6	55.6	289-328 bp	1.0	49.5
ycf1_p4	GCAAAGCGTATAGTCTCCCA	TCAAAAGCAAGAAAAGCAAGAA	50.0	31.8	57.4	55.6	257-296 bp	1.0	48.7
ycf1_p5	CGGCTCCAATTCTTCCTTCT	ATCAAAAGCAAGAAAAGCAAGA	50.0	31.8	57.6	55.4	290-329 bp	1.0	48.2