Hotspot

ndhF-rpl32

SSC · 122769-123865 · 1097 bp

Back to Ceratostigma

Pi 0.0177

MarkerSeek score 71.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1097
variable_sites: 28
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGGTATGCTCCATGCGT	TGTTTCCGAAAAGCCCTTTC	45.0	45.0	57.9	56.8	1201-1220 bp	1.0	73.0
ndhF-rpl32_p2	AAAAGGTATGCTCCATGCGT	TGTTTCCGAAAAGCCCTTTCT	45.0	42.9	57.9	58.3	1201-1220 bp	1.0	71.6
ndhF-rpl32_p3	AAAAGGTATGCTCCATGCGT	ACGCTTTTTGGAAATTGACGT	45.0	38.1	57.9	57.6	1159-1178 bp	1.0	71.0
ndhF-rpl32_p4	AAAAGGTATGCTCCATGCGT	GTTTCCGAAAAGCCCTTTCT	45.0	45.0	57.9	56.5	1200-1219 bp	1.0	70.6
ndhF-rpl32_p5	AAAAGGTATGCTCCATGCGT	ATGTTTCCGAAAAGCCCTTTC	45.0	42.9	57.9	57.3	1202-1221 bp	1.0	69.1