Hotspot

atpH-atpI

LSC · 14213-14929 · 717 bp

Back to Ceratostigma

Pi 0.00651

MarkerSeek score 64.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 717
variable_sites: 7
pi: 0.00651
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	ACAAGTGGTATCCAGGCTCT	45.0	50.0	58.1	58.0	821-825 bp	1.0	80.3
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	ATAGGGGAATCCATGGAGGG	45.0	55.0	58.1	57.9	773-777 bp	1.0	80.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ACAAGTGGTATCCAGGCTCT	50.0	50.0	57.9	58.0	879-883 bp	1.0	80.1
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	50.0	55.0	57.9	57.9	831-835 bp	1.0	80.0
atpH-atpI_p5	AGCCAATCCAGCAGCAATAA	ACAAGTGGTATCCAGGCTCT	45.0	50.0	57.9	58.0	839-843 bp	1.0	79.9