Hotspot

trnK-UUU-rps16

LSC · 4252-5572 · 1321 bp

Back to Ceratostigma

Pi 0.00664
MarkerSeek score 65.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1321
variable_sites
13
pi
0.00664
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AACTCTACCGATGGTGTGGA TCTTGAAAAGGGTGCTCAACC 50.0 47.6 58.1 58.7 1496-1510 bp 1.0 68.0
trnK-UUU-rps16_p2 CGATGGTGTGGACAAATCCT TCTTGAAAAGGGTGCTCAACC 50.0 47.6 57.9 58.7 1488-1502 bp 1.0 67.7
trnK-UUU-rps16_p3 ACCGATGGTGTGGACAAATC TCTTGAAAAGGGTGCTCAACC 50.0 47.6 58.2 58.7 1490-1504 bp 1.0 67.2
trnK-UUU-rps16_p4 AACTCTACCGATGGTGTGGA TCTTGAAAAGGGTGCTCAAC 50.0 45.0 58.1 56.2 1496-1510 bp 1.0 66.9
trnK-UUU-rps16_p5 CGATGGTGTGGACAAATCCT TCTTGAAAAGGGTGCTCAAC 50.0 45.0 57.9 56.2 1488-1502 bp 1.0 66.6