primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AACTCTACCGATGGTGTGGA	TCTTGAAAAGGGTGCTCAACC	20	21	50.0	47.6	58.1	58.7	0.0	0.0	1.760131	4252	5572	1496-1510	1.000000	68.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	CGATGGTGTGGACAAATCCT	TCTTGAAAAGGGTGCTCAACC	20	21	50.0	47.6	57.9	58.7	0.0	0.0	1.807013	4252	5572	1488-1502	1.000000	67.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	ACCGATGGTGTGGACAAATC	TCTTGAAAAGGGTGCTCAACC	20	21	50.0	47.6	58.2	58.7	0.0	0.0	1.871936	4252	5572	1490-1504	1.000000	67.2
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AACTCTACCGATGGTGTGGA	TCTTGAAAAGGGTGCTCAAC	20	20	50.0	45.0	58.1	56.2	0.0	0.0	1.900507	4252	5572	1496-1510	1.000000	66.9
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CGATGGTGTGGACAAATCCT	TCTTGAAAAGGGTGCTCAAC	20	20	50.0	45.0	57.9	56.2	0.0	0.0	1.947389	4252	5572	1488-1502	1.000000	66.6
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TCCACACCAGATCATGTCCT	TTTGGTCCCGCTATTCGAAG	20	20	50.0	50.0	58.0	58.0	0.0	3.3	0.061673	6737	7329	705-1215	1.000000	80.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCCACACCAGATCATGTCCT	TACATTTTAGTGGGGCGTGG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.159958	6737	7329	746-1256	1.000000	79.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TCCACACCAGATCATGTCCT	GTTTTGGTCCCGCTATTCGA	20	20	50.0	50.0	58.0	58.3	0.0	0.0	0.313848	6737	7329	707-1217	1.000000	78.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACACACGTTGCTTTCTACCA	TTTGGTCCCGCTATTCGAAG	20	20	45.0	50.0	57.7	58.0	0.0	3.3	0.337678	6737	7329	680-1190	1.000000	78.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	ACACGTTGCTTTCTACCACA	TTTGGTCCCGCTATTCGAAG	20	20	45.0	50.0	57.7	58.0	0.0	3.3	0.337678	6737	7329	678-1188	1.000000	78.0
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ACGGAAAGAGAGGGATTCGA	ATTGGGGATCTAACCGAGGC	20	20	50.0	55.0	58.2	59.2	0.0	0.0	1.385838	8513	9472	1098-1135	1.000000	73.3
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CGAATCACTCGTACAACGGA	ATTGGGGATCTAACCGAGGC	20	20	50.0	55.0	57.7	59.2	0.0	0.0	1.486359	8513	9472	1068-1105	1.000000	72.7
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	ACGGAAAGAGAGGGATTCGA	AATTGGGGATCTAACCGAGG	20	20	50.0	50.0	58.2	56.7	0.0	0.0	1.476514	8513	9472	1099-1136	1.000000	72.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	CGAATCACTCGTACAACGGA	AATTGGGGATCTAACCGAGG	20	20	50.0	50.0	57.7	56.7	0.0	0.0	1.577035	8513	9472	1069-1106	1.000000	71.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	AAATTGGGGATCTAACCGAGG	20	21	50.0	47.6	58.2	57.4	0.0	0.0	1.729153	8513	9472	1100-1137	1.000000	70.6
atpH-atpI_p1	atpH-atpI	1	AACAGAAGCGGCAGAAATCA	ACAAGTGGTATCCAGGCTCT	20	20	45.0	50.0	58.1	58.0	1.4	0.0	0.145492	14213	14929	821-825	1.000000	80.3
atpH-atpI_p2	atpH-atpI	2	AACAGAAGCGGCAGAAATCA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.1	57.9	1.4	21.3	0.169579	14213	14929	773-777	1.000000	80.2
atpH-atpI_p3	atpH-atpI	3	TACCTTGACCAACTCCAGGT	ACAAGTGGTATCCAGGCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.153991	14213	14929	879-883	1.000000	80.1
atpH-atpI_p4	atpH-atpI	4	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	14213	14929	831-835	1.000000	80.0
atpH-atpI_p5	atpH-atpI	5	AGCCAATCCAGCAGCAATAA	ACAAGTGGTATCCAGGCTCT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.190157	14213	14929	839-843	1.000000	79.9
ycf3_p1	ycf3	1	TCCCACGAGAGGTAATGAGA	TGATTGATTGGCTCTTCCCG	20	20	50.0	50.0	57.2	58.0	0.0	0.0	0.863414	57306	59708	2458-2510	1.000000	78.9
ycf3_p2	ycf3	2	TCCCACGAGAGGTAATGAGA	GATTGGCTCTTCCCGGAATT	20	20	50.0	50.0	57.2	57.9	0.0	0.7	0.879321	57306	59708	2453-2505	1.000000	78.8
ycf3_p3	ycf3	3	TCCCACGAGAGGTAATGAGA	TTGGCTCTTCCCGGAATTTT	20	20	50.0	45.0	57.2	57.7	0.0	0.7	1.123315	57306	59708	2451-2503	1.000000	76.9
ycf3_p4	ycf3	4	TCCCACGAGAGGTAATGAGAA	TGATTGATTGGCTCTTCCCG	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.138598	57306	59708	2458-2510	1.000000	76.7
ycf3_p5	ycf3	5	TTCCCACGAGAGGTAATGAGA	TGATTGATTGGCTCTTCCCG	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.138598	57306	59708	2459-2511	1.000000	76.7
ycf3-accD_p1	ycf3-accD	1	TGAAATTGCAGAGGCTTGGT	GGGGAGTTGTTGGGACTTTT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.082492	59709	60680	1235-1299	1.000000	89.9
ycf3-accD_p2	ycf3-accD	2	TGAAACTCATGCACCTTGCT	GGGGAGTTGTTGGGACTTTT	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.096667	59709	60680	1442-1502	1.000000	89.0
ycf3-accD_p3	ycf3-accD	3	TGAAATTGCAGAGGCTTGGT	GGGAATTGCTAGGGGAGTTG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.058945	59709	60680	1278-1310	0.666667	75.8
ycf3-accD_p4	ycf3-accD	4	TGAAACTCATGCACCTTGCT	GGGAATTGCTAGGGGAGTTG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.073120	59709	60680	1485-1513	0.666667	75.0
ycf3-accD_p5	ycf3-accD	5	GCTGAAGGTGGGACAAAAGA	GGGAATTGCTAGGGGAGTTG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.077336	59709	60680	1549-1577	0.666667	74.8
accD_p1	accD	1	ACGTTACGGTTGGTTATTTGT	CTTAGTCAGCCTTCGATGCA	21	20	38.1	50.0	56.1	57.7	10.2	9.7	3.185063	60681	63356	2667-2901	0.666667	49.9
accD_p2	accD	2	ACGTTACGGTTGGTTATTTGT	ACTTAGTCAGCCTTCGATGC	21	20	38.1	50.0	56.1	57.7	10.2	0.0	3.186647	60681	63356	2668-2902	0.666667	49.9
accD_p3	accD	3	ACGTTACGGTTGGTTATTTGT	AGTCAGCCTTCGATGCATTG	21	20	38.1	50.0	56.1	58.6	10.2	7.7	3.508527	60681	63356	2664-2898	0.666667	47.3
accD_p4	accD	4	ACGTTACGGTTGGTTATTTGT	GTCAGCCTTCGATGCATTGA	21	20	38.1	50.0	56.1	58.6	10.2	12.9	3.512487	60681	63356	2663-2897	0.666667	47.3
accD_p5	accD	5	ACGTTACGGTTGGTTATTTGT	TCAGCCTTCGATGCATTGAC	21	20	38.1	50.0	56.1	58.6	10.2	16.5	3.512487	60681	63356	2662-2896	0.666667	47.3
rpl33-rps18_p1	rpl33-rps18	1	AAGAATCGACACAACACCCC	TACGAAAAGCGCGCTTAGAT	20	20	50.0	45.0	58.1	58.1	0.0	43.0	0.190518	71998	72377	495-527	1.000000	83.1
rpl33-rps18_p2	rpl33-rps18	2	AAGAATCGACACAACACCCC	TAAGGAGGGATCGCTTGGAT	20	20	50.0	50.0	58.1	57.9	0.0	14.2	0.210944	71998	72377	474-506	1.000000	83.0
rpl33-rps18_p3	rpl33-rps18	3	AAGAATCGACACAACACCCC	TTAAGGAGGGATCGCTTGGA	20	20	50.0	50.0	58.1	58.1	0.0	14.2	0.233661	71998	72377	475-507	1.000000	82.8
rpl33-rps18_p4	rpl33-rps18	4	AAGAATCGACACAACACCCC	CGAAAAGCGCGCTTAGATTT	20	20	50.0	45.0	58.1	57.8	0.0	43.0	0.267257	71998	72377	493-525	1.000000	82.5
rpl33-rps18_p5	rpl33-rps18	5	CCCCAGTCGGTTGGAATTAA	TACGAAAAGCGCGCTTAGAT	20	20	50.0	45.0	57.5	58.1	7.6	43.0	0.583120	71998	72377	478-510	1.000000	80.0
rpl2-trnI-CAU_p1	rpl2-trnI-CAU	1	TTGTCCACCCTTTCCAAGTG	GCTAAGCATCCATGGCTGAA	20	20	50.0	50.0	57.9	58.3	0.0	19.8	0.381955	88500	89787	1384-1424	1.000000	80.7
rpl2-trnI-CAU_p2	rpl2-trnI-CAU	2	GCAATCAGTTTCGCTACAGC	GCTAAGCATCCATGGCTGAA	20	20	50.0	50.0	57.8	58.3	0.0	19.8	0.481339	88500	89787	1422-1462	1.000000	79.8
rpl2-trnI-CAU_p3	rpl2-trnI-CAU	3	ATCAGTTTCGCTACAGCACC	GCTAAGCATCCATGGCTGAA	20	20	50.0	50.0	58.3	58.3	1.6	19.8	0.589203	88500	89787	1419-1459	1.000000	78.9
rpl2-trnI-CAU_p4	rpl2-trnI-CAU	4	CCACCCTTTCCAAGTGTGAT	GCTAAGCATCCATGGCTGAA	20	20	50.0	50.0	57.7	58.3	1.2	19.8	0.608885	88500	89787	1380-1420	1.000000	78.9
rpl2-trnI-CAU_p5	rpl2-trnI-CAU	5	TTGTCCACCCTTTCCAAGTG	GCACCCAACTCATAATTGGC	20	20	50.0	50.0	57.9	57.4	0.0	0.0	0.650391	88500	89787	1356-1396	1.000000	78.6
ndhF-rpl32_p1	ndhF-rpl32	1	AAAAGGTATGCTCCATGCGT	TGTTTCCGAAAAGCCCTTTC	20	20	45.0	45.0	57.9	56.8	0.0	0.0	1.297966	122769	123865	1201-1220	1.000000	73.0
ndhF-rpl32_p2	ndhF-rpl32	2	AAAAGGTATGCTCCATGCGT	TGTTTCCGAAAAGCCCTTTCT	20	21	45.0	42.9	57.9	58.3	0.0	0.0	1.467052	122769	123865	1201-1220	1.000000	71.6
ndhF-rpl32_p3	ndhF-rpl32	3	AAAAGGTATGCTCCATGCGT	ACGCTTTTTGGAAATTGACGT	20	21	45.0	38.1	57.9	57.6	0.0	0.0	1.559103	122769	123865	1159-1178	1.000000	71.0
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGGTATGCTCCATGCGT	GTTTCCGAAAAGCCCTTTCT	20	20	45.0	45.0	57.9	56.5	0.0	0.0	1.596187	122769	123865	1200-1219	1.000000	70.6
ndhF-rpl32_p5	ndhF-rpl32	5	AAAAGGTATGCTCCATGCGT	ATGTTTCCGAAAAGCCCTTTC	20	21	45.0	42.9	57.9	57.3	0.0	0.0	1.789271	122769	123865	1202-1221	1.000000	69.1
ccsA-ndhD_p1	ccsA-ndhD	1	GGATATGCTACTTTGGGGTCA	ATCAGTTGATAAGGCGGAAGC	21	21	47.6	47.6	57.1	58.4	0.0	0.0	3.291316	125956	126182	352-353	1.000000	57.1
ccsA-ndhD_p2	ccsA-ndhD	2	TGGATATGCTACTTTGGGGTC	ATCAGTTGATAAGGCGGAAGC	21	21	47.6	47.6	57.1	58.4	0.0	0.0	3.291316	125956	126182	353-354	1.000000	57.1
ccsA-ndhD_p3	ccsA-ndhD	3	TGGATATGCTACTTTGGGGT	ATCAGTTGATAAGGCGGAAGC	20	21	45.0	47.6	55.9	58.4	0.0	0.0	3.556296	125956	126182	353-354	1.000000	54.9
ccsA-ndhD_p4	ccsA-ndhD	4	GGATATGCTACTTTGGGGTCAA	ATCAGTTGATAAGGCGGAAGC	22	21	45.5	47.6	57.8	58.4	0.0	0.0	3.599001	125956	126182	352-353	1.000000	54.6
ccsA-ndhD_p5	ccsA-ndhD	5	TTGGATATGCTACTTTGGGGTC	ATCAGTTGATAAGGCGGAAGC	22	21	45.5	47.6	57.8	58.4	0.0	0.0	3.599001	125956	126182	354-355	1.000000	54.6
ycf1_p1	ycf1	1	CGGCTCCAATTCTTCCTTCT	TCAAAAGCAAGAAAAGCAAGA	20	21	50.0	33.3	57.6	54.8	0.0	0.0	4.621435	134824	134943	289-328	1.000000	51.2
ycf1_p2	ycf1	2	GCAAAGCGTATAGTCTCCCA	TCAAAAGCAAGAAAAGCAAGA	20	21	50.0	33.3	57.4	54.8	0.0	0.0	4.808390	134824	134943	257-296	1.000000	50.2
ycf1_p3	ycf1	3	CGGCTCCAATTCTTCCTTCT	TCAAAAGCAAGAAAAGCAAGAA	20	22	50.0	31.8	57.6	55.6	0.0	0.0	4.833060	134824	134943	289-328	1.000000	49.5
ycf1_p4	ycf1	4	GCAAAGCGTATAGTCTCCCA	TCAAAAGCAAGAAAAGCAAGAA	20	22	50.0	31.8	57.4	55.6	0.0	0.0	5.020015	134824	134943	257-296	1.000000	48.7
ycf1_p5	ycf1	5	CGGCTCCAATTCTTCCTTCT	ATCAAAAGCAAGAAAAGCAAGA	20	22	50.0	31.8	57.6	55.4	0.0	0.0	5.046468	134824	134943	290-329	1.000000	48.2
ycf2-trnI-CAU_p1	ycf2-trnI-CAU	1	CAGAATTCTTGACCCCTCTCA	ATTGGTGTGCATCCAGTAGG	21	20	47.6	50.0	57.0	57.6	4.9	12.2	2.414119	164170	164497	502-506	1.000000	62.0
ycf2-trnI-CAU_p2	ycf2-trnI-CAU	2	ACTCAGAATTCTTGACCCCTC	ATTGGTGTGCATCCAGTAGG	21	20	47.6	50.0	57.0	57.6	4.9	12.2	2.416102	164170	164497	505-509	1.000000	61.9
ycf2-trnI-CAU_p3	ycf2-trnI-CAU	3	CTCAGAATTCTTGACCCCTCTC	ATTGGTGTGCATCCAGTAGG	22	20	50.0	50.0	57.9	57.6	4.9	12.2	2.560890	164170	164497	504-508	1.000000	60.8
ycf2-trnI-CAU_p4	ycf2-trnI-CAU	4	CAGAATTCTTGACCCCTCTCA	GCCAATTATGAGTTGGGTGC	21	20	47.6	50.0	57.0	57.4	4.9	7.0	2.573468	164170	164497	464-468	1.000000	60.8
ycf2-trnI-CAU_p5	ycf2-trnI-CAU	5	ACTCAGAATTCTTGACCCCTC	GCCAATTATGAGTTGGGTGC	21	20	47.6	50.0	57.0	57.4	4.9	7.0	2.575450	164170	164497	467-471	1.000000	60.8