Hotspot

ycf3

LSC · 41329-43304 · 1976 bp

Back to Ceratopteris

Pi 0.14

MarkerSeek score 82.10

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1976
variable_sites: 388
pi: 0.14
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AGCAACAACCTACCAACAGG	TCCGGTGCTATGCCTTAAAC	50.0	50.0	58.0	58.0	2535-2564 bp	1.0	91.6
ycf3_p2	AGCAACAACCTACCAACAGG	AGGAAAGGGAGGGATTCGAA	50.0	50.0	58.0	58.0	2585-2614 bp	1.0	91.6
ycf3_p3	GCAACAACCTACCAACAGGA	TCCGGTGCTATGCCTTAAAC	50.0	50.0	58.0	58.0	2534-2563 bp	1.0	91.5
ycf3_p4	GCAACAACCTACCAACAGGA	AGGAAAGGGAGGGATTCGAA	50.0	50.0	58.0	58.0	2584-2613 bp	1.0	91.5
ycf3_p5	AGCAACAACCTACCAACAGG	GAAAGGGAGGGATTCGAACC	50.0	55.0	58.0	58.0	2583-2612 bp	1.0	91.5