Hotspot

rpl2

LSC · 80352-81927 · 1576 bp

Back to Ceratopteris

Pi 0.16

MarkerSeek score 82.90

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 1576
variable_sites: 356
pi: 0.16
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CTACCCAAACCTCACCGATG	TGGCGAATTCACAGGTTCAA	55.0	45.0	58.0	58.0	2721 bp	0.333333	52.9
rpl2_p2	CTACCCAAACCTCACCGATG	TTGGCGAATTCACAGGTTCA	55.0	45.0	58.0	58.0	2722 bp	0.333333	52.9
rpl2_p3	AACTATTGCAGAAGAGCGGG	TGGCGAATTCACAGGTTCAA	50.0	45.0	58.0	58.0	2295 bp	0.333333	52.9
rpl2_p4	AACTATTGCAGAAGAGCGGG	TTGGCGAATTCACAGGTTCA	50.0	45.0	58.0	58.0	2296 bp	0.333333	52.9
rpl2_p5	TTCCTTCTCAAAGCTTGCGT	TGGCGAATTCACAGGTTCAA	45.0	45.0	58.0	58.0	2518 bp	0.333333	52.9