Hotspot

petB

LSC · 71072-72572 · 1501 bp

Back to Ceratopteris

Pi 0.136

MarkerSeek score 83.30

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1501
variable_sites: 290
pi: 0.136
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGCATAACCGGTGAAGCTAA	GACGGCTATTGCTCCAAAGA	50.0	50.0	58.0	58.0	2517 bp	0.333333	52.9
petB_p2	GGCTGACCAAAAGCGGTATA	GACGGCTATTGCTCCAAAGA	50.0	50.0	58.0	58.0	2540 bp	0.333333	52.9
petB_p3	TAAGGCATAACCGGTGAAGC	GACGGCTATTGCTCCAAAGA	50.0	50.0	58.0	58.0	2520 bp	0.333333	52.9
petB_p4	CAAGTGAACGGGATATGGGG	GACGGCTATTGCTCCAAAGA	55.0	50.0	58.0	58.0	2497 bp	0.333333	52.9
petB_p5	GGCATAACCGGTGAAGCTAA	TTTTACCCCGACGGCTATTG	50.0	50.0	58.0	58.0	2526 bp	0.333333	52.9