Hotspot

rps4-trnL-UAA

LSC · 44502-45270 · 769 bp

Back to Ceratopteris

Pi 0.234

MarkerSeek score 82.60

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 245
pi: 0.234
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	AACGCATCTCAAGCAATTGC	CAGAATGTCAGTAGCTGCGT	45.0	50.0	58.0	58.0	1632 bp	0.333333	53.2
rps4-trnL-UAA_p2	CGTGGAAGCCATACCTAACC	CAGAATGTCAGTAGCTGCGT	55.0	50.0	58.1	58.0	1670 bp	0.333333	52.9
rps4-trnL-UAA_p3	GTGGAAGCCATACCTAACCG	CAGAATGTCAGTAGCTGCGT	55.0	50.0	58.1	58.0	1669 bp	0.333333	52.9
rps4-trnL-UAA_p4	AACGCATCTCAAGCAATTGC	GGTACGAGATTTGGCTCAGG	45.0	55.0	58.0	58.1	1155 bp	0.333333	52.8
rps4-trnL-UAA_p5	TGAACCCCTGGTTTTTCTGG	CAGAATGTCAGTAGCTGCGT	50.0	50.0	57.9	58.0	1595 bp	0.333333	52.7