Hotspot

psbM-petN

LSC · 27848-29790 · 1943 bp

Back to Ceratopteris

Pi 0.265

MarkerSeek score 69.30

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 1943
variable_sites: 247
pi: 0.265
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.32
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TTACATTCAGACAGCCGCTC	TCTTTCGCTTGTAGTTCGGG	50.0	50.0	58.0	57.9	2014 bp	0.333333	52.7
psbM-petN_p2	GGGGGCTGGATTACTCAATG	TCTTTCGCTTGTAGTTCGGG	55.0	50.0	58.0	57.9	2518 bp	0.333333	52.6
psbM-petN_p3	CAGCCGCTCAGAGTAACAAT	TCTTTCGCTTGTAGTTCGGG	50.0	50.0	58.0	57.9	2003 bp	0.333333	52.6
psbM-petN_p4	TTCTTGCGTTTGTAGCCACT	TCTTTCGCTTGTAGTTCGGG	45.0	50.0	58.0	57.9	2075 bp	0.333333	52.4
psbM-petN_p5	TTACATTCAGACAGCCGCTC	AGGAAAAGGCGTGAATCTCC	50.0	50.0	58.0	57.9	2278 bp	0.333333	52.3