Hotspot

trnS-GCU; trnS-GGA; trnS-UGA-ycf12

LSC · 7466-8184 · 719 bp

Back to Ceratopteris

Pi 0.265
MarkerSeek score 62.20
Primer available yes
Label trnS-GCU; trnS-GGA; trnS-UGA-ycf12

Feature Metadata

label_name
trnS-GCU; trnS-GGA; trnS-UGA-ycf12
feature_type
igs
region
LSC
length_bp
719
variable_sites
183
pi
0.265
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.64
markerseek_score
62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU; trnS-GGA; trnS-UGA-ycf12_p1 TCGATGCAACGGCGTATTAA GAGTCGTTGAAGCACTGGAT 45.0 50.0 58.1 57.9 2005 bp 0.333333 52.0
trnS-GCU; trnS-GGA; trnS-UGA-ycf12_p2 TCGATGCAACGGCGTATTAA AAAGCCTCAGAAATTGCGGA 45.0 45.0 58.1 58.1 1441 bp 0.333333 52.0
trnS-GCU; trnS-GGA; trnS-UGA-ycf12_p3 TCGATGCAACGGCGTATTAA AAGCCTCAGAAATTGCGGAA 45.0 45.0 58.1 58.1 1440 bp 0.333333 52.0
trnS-GCU; trnS-GGA; trnS-UGA-ycf12_p4 TAGCAATCCGTCGCTTTCAA GAGTCGTTGAAGCACTGGAT 45.0 50.0 57.9 57.9 1449 bp 0.333333 51.9
trnS-GCU; trnS-GGA; trnS-UGA-ycf12_p5 TTAGCAATCCGTCGCTTTCA GAGTCGTTGAAGCACTGGAT 45.0 50.0 57.9 57.9 1450 bp 0.333333 51.9