Hotspot

psbK-psbI

LSC · 6668-7203 · 536 bp

Back to Ceratopteris

Pi 0.283

MarkerSeek score 67.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 204
pi: 0.283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTACCCGAAGCTTATGCCA	AGAGGACGATAGAAGGGAGC	50.0	55.0	58.0	58.0	1123 bp	0.333333	53.0
psbK-psbI_p2	GTTACCCGAAGCTTATGCCA	GAGGACGATAGAAGGGAGCT	50.0	55.0	58.0	58.0	1122 bp	0.333333	53.0
psbK-psbI_p3	GTTACCCGAAGCTTATGCCA	CTAGCGTTCCCAAGCAGATT	50.0	50.0	58.0	58.0	1355 bp	0.333333	52.8
psbK-psbI_p4	GTTACCCGAAGCTTATGCCA	GACTCAATCCTTACGGCCAG	50.0	55.0	58.0	58.1	762 bp	0.333333	52.6
psbK-psbI_p5	TAACCGCGTCGAGGAAAAAT	GAGGACGATAGAAGGGAGCT	45.0	55.0	57.9	58.0	1234 bp	0.333333	52.6