Hotspot

matK-rps16

LSC · 2392-3098 · 707 bp

Back to Ceratopteris

Pi 0.243

MarkerSeek score 81.80

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 707
variable_sites: 233
pi: 0.243
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	TAAGTTCGCGCACGTTATCA	CGAATAGTCGCAATTGACGC	45.0	50.0	58.0	58.0	1128 bp	0.333333	53.1
matK-rps16_p2	TAAGTTCGCGCACGTTATCA	GAGTATAGGGCGCTGATTCG	45.0	55.0	58.0	58.0	1377 bp	0.333333	53.0
matK-rps16_p3	TAAGTTCGCGCACGTTATCA	GGAAGGTCACTAGGGAGGTT	45.0	55.0	58.0	58.0	1091 bp	0.333333	52.8
matK-rps16_p4	TAAGTTCGCGCACGTTATCA	GGGGTTAATCTCTGCATGCA	45.0	50.0	58.0	57.9	1170 bp	0.333333	52.7
matK-rps16_p5	TAAGTTCGCGCACGTTATCA	TTGGGTTTTACAATCCCCGG	45.0	50.0	58.0	58.1	1073 bp	0.333333	52.6