Hotspot

rpl32-trnL-UAG

SSC · 114973-115998 · 1026 bp

Back to Ceratophyllum

Pi 0.0401
MarkerSeek score 78.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1026
variable_sites
73
pi
0.0401
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TTTTTGTGCGACAAACGAGT AGCGTGTCTACCGATTTCAC 40.0 50.0 57.1 58.0 1030-1093 bp 1.0 82.2
rpl32-trnL-UAG_p2 TTTTTGTGCGACAAACGAGT CAGCGTGTCTACCGATTTCA 40.0 50.0 57.1 58.0 1031-1094 bp 1.0 82.2
rpl32-trnL-UAG_p3 TTTTTGTGCGACAAACGAGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.1 57.9 1040-1103 bp 1.0 81.2
rpl32-trnL-UAG_p4 TTTTTGTGCGACAAACGAGT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 57.1 57.8 1039-1102 bp 1.0 80.7
rpl32-trnL-UAG_p5 TTTTTGTGCGACAAACGAGT CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.1 57.8 1038-1101 bp 1.0 80.7