Hotspot

ndhF-rpl32

SSC · 114178-114798 · 621 bp

Back to Ceratophyllum

Pi 0.0323

MarkerSeek score 72.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 621
variable_sites: 39
pi: 0.0323
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGTATGATCCACGCATATTGA	GTACGTTTCTTTGGAACCGC	45.5	50.0	58.1	57.7	675-680 bp	1.0	68.1
ndhF-rpl32_p2	GGGTATGATCCACGCATATTG	GTACGTTTCTTTGGAACCGC	47.6	50.0	56.6	57.7	675-680 bp	1.0	65.6
ndhF-rpl32_p3	GGGTATGATCCACGCATATTGA	TACGTTTCTTTGGAACCGCC	45.5	50.0	58.1	58.8	674-679 bp	1.0	64.4
ndhF-rpl32_p4	GGGTATGATCCACGCATATTG	TACGTTTCTTTGGAACCGCC	47.6	50.0	56.6	58.8	674-679 bp	1.0	61.9
ndhF-rpl32_p5	GGGTATGATCCACGCATATT	GTACGTTTCTTTGGAACCGC	45.0	50.0	55.1	57.7	675-680 bp	1.0	61.1