Hotspot

ndhC-trnV-UAC

LSC · 52138-53490 · 1353 bp

Back to Ceratophyllum

Pi 0.0242
MarkerSeek score 75.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1353
variable_sites
64
pi
0.0242
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1438-1484 bp 1.0 84.3
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT GCCCAATGCAACAAAAATGC 50.0 45.0 57.9 57.7 1520-1566 bp 1.0 82.9
ndhC-trnV-UAC_p3 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1437-1483 bp 1.0 81.6
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1426-1472 bp 1.0 80.5
ndhC-trnV-UAC_p5 GGGGCTAAAACTCCGGAAAT AAGGTCTACGGTTCGAGTCC 50.0 55.0 57.9 58.8 1436-1482 bp 1.0 79.3