Hotspot

trnF-GAA-ndhJ

LSC · 50054-50442 · 389 bp

Back to Ceratophyllum

Pi 0.0257

MarkerSeek score 66.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 389
variable_sites: 18
pi: 0.0257
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGCCCCCGATTTCTATGAAA	55.0	45.0	58.0	56.9	469-497 bp	1.0	78.0
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	TTGCCCCCGATTTCTATGAA	55.0	45.0	58.0	56.9	470-498 bp	1.0	78.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGCCCCCGATTTCTATGAAA	50.0	45.0	58.1	56.9	433-461 bp	1.0	77.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGCCCCCGATTTCTATGAA	50.0	45.0	58.1	56.9	434-462 bp	1.0	77.9
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	TGCCCCCGATTTCTATGAAA	55.0	45.0	57.9	56.9	457-485 bp	1.0	77.0