Pi
0.0304
MarkerSeek score
70.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1091-1101 bp | 1.0 | 85.5 |
| trnT-UGU-trnL-UAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 1122-1132 bp | 1.0 | 85.1 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1099-1109 bp | 1.0 | 84.9 |
| trnT-UGU-trnL-UAA_p4 | GAGTCGAACCGATGACCATC | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 58.2 | 58.1 | 1130-1140 bp | 1.0 | 84.5 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | AGTCCGTAGCGTCTACCAAT | 55.0 | 50.0 | 57.9 | 58.2 | 1105-1115 bp | 1.0 | 83.5 |