Hotspot

rpoB-trnC-GCA

LSC · 27159-28255 · 1097 bp

Back to Ceratophyllum

Pi 0.0123
MarkerSeek score 64.00
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1097
variable_sites
27
pi
0.0123
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGTCAAGCCCTGATCAAGAA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1252-1267 bp 1.0 80.5
rpoB-trnC-GCA_p2 TGAAGTTCTTCCGTCAAGCC CCGGATTTGAACTGGGGAAA 50.0 50.0 58.1 58.1 1263-1278 bp 1.0 80.3
rpoB-trnC-GCA_p3 CGTCAAGCCCTGATCAAGAA AGTCTTGTGTTGATCAGGCG 50.0 50.0 57.9 58.2 1276-1291 bp 1.0 79.5
rpoB-trnC-GCA_p4 CGTCAAGCCCTGATCAAGAA GTCTTGTGTTGATCAGGCGA 50.0 50.0 57.9 58.2 1275-1290 bp 1.0 79.5
rpoB-trnC-GCA_p5 CGTCAAGCCCTGATCAAGAA TCTTGTGTTGATCAGGCGAC 50.0 50.0 57.9 58.2 1274-1289 bp 1.0 79.5