Hotspot

atpH-atpI

LSC · 14083-14915 · 833 bp

Back to Ceratophyllum

Pi 0.018

MarkerSeek score 60.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 28
pi: 0.018
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	929-941 bp	1.0	77.1
atpH-atpI_p2	GCAGTACCTTGACCAACTCC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.2	58.5	981-993 bp	1.0	76.8
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	936-948 bp	1.0	76.7
atpH-atpI_p4	TACCTTCTACGGCTTGACCT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1004-1016 bp	1.0	76.4
atpH-atpI_p5	GCCAATAGAAGCAAGGCCTA	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.7	58.5	958-970 bp	1.0	75.6