Hotspot

trnS-GCU-trnG-UCC

LSC · 8469-9272 · 804 bp

Back to Ceratophyllum

Pi 0.0293
MarkerSeek score 74.60
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
804
variable_sites
46
pi
0.0293
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA AATCAAACCGAAAGACCCCT 45.0 45.0 58.0 57.0 921-1000 bp 1.0 78.2
trnS-GCU-trnG-UCC_p2 ATTAGCAATCCGCCGCTTTA ATCAAACCGAAAGACCCCTT 45.0 45.0 58.0 57.0 920-999 bp 1.0 78.2
trnS-GCU-trnG-UCC_p3 GATCAGAGGGAACGGAGAGA ATCAAACCGAAAGACCCCTT 55.0 45.0 57.9 57.0 980-1059 bp 1.0 77.7
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA AATCAAACCGAAAGACCCCT 55.0 45.0 57.9 57.0 981-1060 bp 1.0 77.6
trnS-GCU-trnG-UCC_p5 AAGTGATCAGAGGGAACGGA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.1 57.0 984-1063 bp 1.0 77.6