Hotspot

psbK-psbI

LSC · 7648-8105 · 458 bp

Back to Ceratophyllum

Pi 0.0192

MarkerSeek score 62.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 458
variable_sites: 15
pi: 0.0192
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCCTCACGTCCAGGATTACG	50.0	55.0	57.9	58.9	583-595 bp	1.0	76.0
psbK-psbI_p2	GCCAGTCATTCCTTTGCTCT	TCCTCACGTCCAGGATTACG	50.0	55.0	58.2	58.9	620-632 bp	1.0	74.9
psbK-psbI_p3	ATGCCAGTCATTCCTTTGCT	TCCTCACGTCCAGGATTACG	45.0	55.0	57.8	58.9	622-634 bp	1.0	74.4
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	580-592 bp	1.0	74.0
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TCCTCACGTCCAGGATTACG	45.0	55.0	58.3	58.9	581-593 bp	1.0	74.0