Hotspot

rps16-trnQ-UUG

LSC · 6205-7018 · 814 bp

Back to Ceratophyllum

Pi 0.032

MarkerSeek score 67.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 51
pi: 0.032
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	820-887 bp	1.0	87.0
rps16-trnQ-UUG_p2	CCTTCAATTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	830-897 bp	1.0	86.2
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	824-891 bp	1.0	85.9
rps16-trnQ-UUG_p4	CCTTCAATTCAAGTCGCACG	CTCGGAGGTTCGAATCCTTC	50.0	55.0	57.8	57.8	834-901 bp	1.0	85.1
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTGACATTTCAATGGGGCGT	45.0	45.0	58.1	58.4	880-947 bp	1.0	84.3