Hotspot

ycf1

SSC · 121393-126609 · 5217 bp

Back to Ceratocephala

Pi 0.00134

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5217
variable_sites: 7
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGGGCCTTCTTTACTCGTG	TCTCAAACTGCCTGGTCAAA	55.0	45.0	58.0	57.3	282 bp	1.0	74.9
ycf1_p2	TCTCGAGGGCCTTCTTTACT	TCTCAAACTGCCTGGTCAAA	50.0	45.0	57.8	57.3	286 bp	1.0	73.2
ycf1_p3	GAGGGCCTTCTTTACTCGTG	ATCTCAAACTGCCTGGTCAA	55.0	45.0	58.0	57.0	283 bp	1.0	73.1
ycf1_p4	GAGGGCCTTCTTTACTCGTG	TCTCAAACTGCCTGGTCAAAA	55.0	42.9	58.0	58.0	282 bp	1.0	72.7
ycf1_p5	AGGGCCTTCTTTACTCGTGA	TCTCAAACTGCCTGGTCAAA	50.0	45.0	58.4	57.3	281 bp	1.0	72.2