Hotspot

ndhK

LSC · 49731-50414 · 684 bp

Back to Ceratocephala

Pi 0.00146

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	TGAACAAAATGTCCCGAGCA	AAAAGGGGCATTGGAATGGT	45.0	45.0	58.0	58.0	783 bp	1.0	79.5
ndhK_p2	AAAATGTCCCGAGCATCCTG	AAAAGGGGCATTGGAATGGT	50.0	45.0	58.2	58.0	778 bp	1.0	77.8
ndhK_p3	CAAAATGTCCCGAGCATCCT	AAAAGGGGCATTGGAATGGT	50.0	45.0	58.2	58.0	779 bp	1.0	77.8
ndhK_p4	GAGGTGCCACTCGCTATTTT	AAAAGGGGCATTGGAATGGT	50.0	45.0	58.3	58.0	802 bp	1.0	77.7
ndhK_p5	TGAACAAAATGTCCCGAGCA	CGAAAAGGGGCATTGGAATG	45.0	50.0	58.0	57.7	785 bp	1.0	77.5