Hotspot

atpB

LSC · 53632-55128 · 1497 bp

Back to Ceratocephala

Pi 0.002

MarkerSeek score 59.90

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 3
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	ATTTGACCGCTATTCGTCGA	TCGATCGGGCATCTACTACT	45.0	50.0	57.4	57.4	1616 bp	1.0	71.1
atpB_p2	TTTGACCGCTATTCGTCGAT	TCGATCGGGCATCTACTACT	45.0	50.0	57.4	57.4	1615 bp	1.0	71.1
atpB_p3	ACAGTTCGATTGGGAGTCAG	TCGATCGGGCATCTACTACT	50.0	50.0	57.2	57.4	1565 bp	1.0	69.6
atpB_p4	CAGTTCGATTGGGAGTCAGT	TCGATCGGGCATCTACTACT	50.0	50.0	57.2	57.4	1564 bp	1.0	69.6
atpB_p5	CGATTGGGAGTCAGTACACA	TCGATCGGGCATCTACTACT	50.0	50.0	57.0	57.4	1559 bp	1.0	67.4