Hotspot

ndhF-rpl32

SSC · 110092-110989 · 898 bp

Back to Ceratocephala

Pi 0.00112
MarkerSeek score 60.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
898
variable_sites
1
pi
0.00112
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGAAGACCCACCCCTATT AAAAGGCCTTTATTGCTGCC 55.0 45.0 58.1 57.2 1071-1077 bp 1.0 73.5
ndhF-rpl32_p2 GCTAAAGGAACTGGAAGGGG AAAAGGCCTTTATTGCTGCC 55.0 45.0 57.9 57.2 1051-1057 bp 1.0 73.3
ndhF-rpl32_p3 GAAAAGGAGAAGACCCACCC AAAAGGCCTTTATTGCTGCC 55.0 45.0 57.8 57.2 1076-1082 bp 1.0 72.8
ndhF-rpl32_p4 GGAAAAGGAGAAGACCCACC AAAAGGCCTTTATTGCTGCC 55.0 45.0 57.8 57.2 1077-1083 bp 1.0 72.8
ndhF-rpl32_p5 GGGAAAAGGAGAAGACCCAC AAAAGGCCTTTATTGCTGCC 55.0 45.0 57.8 57.2 1078-1084 bp 1.0 72.8