Hotspot

petA-psbJ

LSC · 63027-63587 · 561 bp

Back to Ceratocephala

Pi 0.00357

MarkerSeek score 60.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 2
pi: 0.00357
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTCGCGTCGGTTATTTTGG	ATTCTGGATTGGGCTCATCC	50.0	50.0	57.8	57.3	675-677 bp	1.0	73.6
petA-psbJ_p2	TTTCTTCTTCGCGTCGGTTA	ATTCTGGATTGGGCTCATCC	45.0	50.0	57.6	57.3	681-683 bp	1.0	71.5
petA-psbJ_p3	TTTTCTTCTTCGCGTCGGTT	ATTCTGGATTGGGCTCATCC	45.0	50.0	58.4	57.3	682-684 bp	1.0	71.5
petA-psbJ_p4	TTTTTCTTCTTCGCGTCGGT	ATTCTGGATTGGGCTCATCC	45.0	50.0	58.4	57.3	683-685 bp	1.0	71.5
petA-psbJ_p5	CTTCGCGTCGGTTATTTTGG	GGCACTGTAGCTGGTATTCT	50.0	50.0	57.8	57.0	733-735 bp	1.0	71.1