Hotspot

rbcL-accD

LSC · 57352-58197 · 846 bp

Back to Ceratocephala

Pi 0.00236

MarkerSeek score 60.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 846
variable_sites: 2
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TGCAAATGGAGCCTTGAACT	CGTCAAATTGAACCCCAACG	45.0	50.0	58.0	58.0	966 bp	1.0	79.8
rbcL-accD_p2	TGCAAATGGAGCCTTGAACT	AGAAGGCCCGTGTACTAACT	45.0	50.0	58.0	58.1	990 bp	1.0	79.6
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	CGTCAAATTGAACCCCAACG	50.0	50.0	57.9	58.0	942 bp	1.0	78.9
rbcL-accD_p4	TGCAAATGGAGCCTTGAACT	TTGAGTAAAGAAGGCCCGTG	45.0	50.0	58.0	57.8	998 bp	1.0	78.7
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	AGAAGGCCCGTGTACTAACT	50.0	50.0	57.9	58.1	966 bp	1.0	78.7